منابع مشابه
Optimal clearing and mounting media for confocal microscopy of thick specimens
Sample preparation for confocal microscopy typically involves fixing and staining, followed by clearing and mounting. Clearing involves treatment with solvent to dissolve lipids and other structures that cause light scattering and reduce the ability to image through thick specimens. Mounting involves transfer of the specimen into a medium which matches the refractive index the objective is desi...
متن کاملMethods for imaging thick specimens: confocal microscopy, deconvolution, and structured illumination.
When a thick specimen is viewed through a conventional microscope, one sees the sum of a sharp image of an in-focus region plus blurred images of all of the out-of-focus regions. High background, scattering, and aberrations are all problems when viewing thick specimens. Several methods are available to deal with these problems in living samples. These methods can be grouped into three classes: ...
متن کاملDeep tissue fluorescent imaging in scattering specimens using confocal microscopy.
In scattering specimens, multiphoton excitation and nondescanned detection improve imaging depth by a factor of 2 or more over confocal microscopy; however, imaging depth is still limited by scattering. We applied the concept of clearing to deep tissue imaging of highly scattering specimens. Clearing is a remarkably effective approach to improving image quality at depth using either confocal or...
متن کاملConfocal Microscopy
A confocal microscope creates sharp images of a specimen that would otherwise appear blurred when viewed with a conventional microscope. This is achieved by excluding most of the light from the specimen that is not from the microscope’s focal plane. The image has less haze and better contrast than that of a conventional microscope and represents a thin cross-section of the specimen. Thus, apart...
متن کاملConfocal microscopy
The confocal microscope, based on the principle of confocal imaging, is a recently introduced and invaluable tool for high-resolution fluorescence microscopy. It has gained popularity in the scientific and industrial communities since its inception. Although the application of confocal microscopes in dentistry started as early as 1994, the practical usage of this device in our country is just g...
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ژورنال
عنوان ژورنال: Journal of Biomedical Optics
سال: 2009
ISSN: 1083-3668
DOI: 10.1117/1.3156813